reducing variability in cell-specific productivity in

Reducing Variability in Cell

Thus it is important to measure variability in productivity performance as cell-specific productivity (q P) which incorporates all three factors (titer cell density and CSPR) A perfusion system is distinct from other culture systems in that it consists of not only a bioreactor but also a cell retention device such as the settler we use (Figure 1B) that retains cell biomass in the

Understanding plant response to nitrogen limitation for

Need for genetic improvement of nitrogen use efficiency (NUE) In the past 50 years there has been a marked increase in food productivity allowing for a significant decrease in world hunger despite a doubling of the population (Godfray et al 2010) However it will be challenging over the next 50 years to increase crop productivity further in order to meet a growing population due to a range

Cell culture processes for monoclonal antibody production

For antibody manufacturing process development maintaining desired quality attributes while reducing time to market maintaining cost effectiveness and providing manufacturing flexibility are key issues in today's competitive market where several companies *Correspondence to: Ashraf Amanullah Email: [email protected] Submitted: 04/14/10 Accepted: 06/19/10 Previously published online

The Fluctuating Cell

Individual cells of cyanobacteria or algae are supplied with light in a highly irregular fashion when grown in industrial-scale photobioreactors (PBRs) These conditions coincide with significant reductions in growth rate compared to the static light environments commonly used in laboratory experiments We grew a dense culture of the model cyanobacterium Synechocystis sp PCC 6803 under a

Animal Cell Expression Systems

However the partition of requirements between these demands depends on the relative values of cell-specific productivity and growth rate both of which can change during the time course of a culture Undoubtedly this type of mathematical framework can lead to an understanding of the specific requirements of nucleotide sugars and allow the development of rational feeding strategies

Embryonic and Induced Pluripotent Stem Cells

or differentiation of embryonic and induced pluripotent stem cells Human Pluripotent Stem Cell Functional Identification Kit • Efficient: differentiation and identification in 5 days • Reliable: induces ES/iPS tri-lineage differentiation with kit-provided supplements • Complete: contains germ layer-specific antibodies to confirm successful differentiation Human Three Germ Layer 3-Color


Since nitrogen (N) is often limiting in permafrost soils we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples TongWein along a permafrost thaw gradient in an Alaskan boreal soil Samples from minimally moderately and extensively thawed sites were TongWein to a depth of 79 cm to encompass zones above and below the

BIOT 1 Understanding and mitigating raw material

Understanding and mitigating raw material variability in an intensified integrated perfusion cell culture process Myles Hollenbach hollenbach mylesoutlook Jonathan Wang Neha Shah Jason Walther Christopher Hwang Late Stage Process Development Sanofi Worcester Massachusetts United States Sanofi's next-generation integrated continuous biomanufacturing platform affords

Katherina Petrou

Katherina Petrouuts edu au Phone +61 2 95144159 0000-0002-2703-0694 Edit your profile Biography I am a senior lecturer in the School of Life Sciences (SoLS) Program Director BSc Marine Biology and leader of the Marine Microphycology Lab (MML) Coupling cell physiology photobiology and biochemistry my research investigates cellular processes biochemical and biomolecular shifts in

Microbial Functioning and Community Structure

We analyzed the regional distribution of bulk heterotrophic prokaryotic activity (leucine incorporation) and selected single-cell parameters (cell viability and nucleic acid content) as parameters for microbial functioning as well as bacterial and archaeal community structure in the epipelagic (0 to 200 m) and mesopelagic (200 to 1 000 m) subtropical Northeast Atlantic Ocean

CHO cells adapted to hypothermic growth produce high

High yields of recombinant proteins can be obtained by a biphasic bioprocess strategy involving a temperature change from a standard temperature (37C) suitable for cell growth to hypothermic conditions in which there is an enhanced cell‐specific productivity (Q p) 11 The ability to attain a high product titer is dependent on the balance of the intrinsic parameters of the cell as well as

Microbial Respiration the Engine of Ocean

Microbial plankton respiration is the key determinant in the balance between the storage of organic carbon in the oceans or its conversion to carbon dioxide with accompanying consumption of dissolved oxygen Over the past 50 years dissolved oxygen concentrations have decreased in many parts of the world's oceans and this trend of ocean deoxygenation is predicted to continue

Shimoni Y 2017 April Reducing variability in cell specific productivity in perfusion culture In Abstracts of papers of the American chemical society (Vol 253) 3 Mollah A H 2005 Application of failure mode and effect analysis (FMEA) for process risk assessment BioProcess Int 3(10) pp 12-20 4 McGillicuddy N 2017 Examining the sources of variability in cell culture media used for

Cell Press: Cell Press

x G protein-coupled receptors (GPCRs) are the most intensively studied drug targets because of their diversity cell-specific expression and druggable sites accessible at the cell surface Preclinical and clinical studies suggest that targeting GPCRs activated by fatty acid-derived lipids may have potential to improve glucose homeostasis and reduce complications in patients with type 2


• Cell specific productivity is higher in High Productivity Harvest process compared to traditional FB process • Higher perfusion rate (1vvd vs 0 5vvd) shows higher cell specific productivity -HP Harvest FB_ATF 1 0 vvd -HP Harvest FB_ATF 0 5 vvd -HP Harvest FB_ATF 0 25 vvd -Traditional FB

Proliferation control strategies to improve productivity

01 03 2007In experiments to compare the effects of growth inhibitors the increase in cell specific productivity following expression of p53175P p21 CIP1 or p27 KIP1 are comparable to each other the improvement in production therefore being the effect of cell cycle arrest rather than due to any specific growth inhibitor (Fussenegger et al 1997)

Combining lipoic acid to methylene blue reduces the

The cell specific oxygen consumption rate (q O2) observed for the control culture at 24 h with q O2 = 0 22 0 02 μmolO 2 /10 6 cells/h was similar to previous data obtained with the same cell line While being maintained during exponential growth phase q O2 then constantly and strongly decreased


02 04 2020Published Wednesday February 7 2018 at 6287 3958 in Reducing Variability in Cell-Specific Productivity in Perfusion Culture: A Case Study Figure 7: In a perfusion system cells constantly shuttle between the bioreactor and the settler the latter being controlled at 20 C lower than the former Lower settler temperature reduces the cells' metabolic rate to help them cope with

Zephyr G3 NGS Workstations

The simplified user-interface and integrated hardware maximize laboratory productivity while reducing variability resulting from manual pipetting Over 40 automated methods for the Zephyr G3 NGS liquid handler are available automating: Post-PCR SPRI purification qPCR setup (in 96-well or 384-well plates)

b Six Sigma focuses on reducing variability in key product

B six sigma focuses on reducing variability in key School Lamar University Course Title INEN 4300 Uploaded By jtorres0319 Pages 6 This preview shows page 3 - 5 out of 6 pages b Six-Sigma focuses on reducing variability in key product quality characteristics to

Towards improving construction labor productivity and

The goal of lean construction should be to improve performance by reducing variability in labor productivity The variability in daily labor productivity for each project can be calculated by using the following equation: (15) Variation (V j) = ∑ (UR ij-baseline productivity) 2 n where UR ij = the daily productivity (unit rate) for workday i on project j and n = the number of workdays on project j

Diminution in Variability of Blockwork Labour Productivity

productivity is more highly correlated to project performance which means that reducing variability in labour productivity appears to have a significant effect on performance Also the performance gap value for block work was found to be 0 374 man hrs/m 2 It was recommended that the site managers should determine to get more output with a reduction in labour input Keywords: Variability

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